Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases

Abstract

Background: Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its tradi­tional diagnosis is based on blood culture and serological methods. For more sensitive and specific detection, the PCR method is recommended. Methods: One hundred four blood samples were gathered from suspicious patients with brucellosis different ages form Ker­manshah, Mazandaran, Khorassan and Hormozgan provinces, and were examined by Rose Bengal, anti globulin, culture and PCR methods. Results: Seventy three samples were positive by PCR method, 15 samples were positive by cultured method and 84 sam­ples were positive by serological methods. Conclusion: PCR method is sensitive and specific for diagnosis of Brucella from peripheral blood in suspected cases.

Keywords

• Brucella
• Culture and serological methods
• PCR