Improvement of triterpenoid production in mycelia of Antrodia camphorata through mutagenesis breeding and amelioration of CCl4-induced liver injury in mice
Huan-Ju Wang,
Ce Cui,
Xiao-Mei Gong,
Shuo Wang,
Cheng-Xi Li,
Hao Guo,
Ya-Ling Wang,
Yu-Dan Huang,
Jian-Lin Jiang,
Xue-Mei Luo,
Jian-Hua Miao,
Tian-Qi Liu,
Shuai Zhao,
Jia-Xun Feng
Affiliations
Huan-Ju Wang
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China
Ce Cui
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China
Xiao-Mei Gong
Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China; School of Life Science, Bengbu Medical College, Bengbu, 233030, China
Hao Guo
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China
Ya-Ling Wang
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China
Yu-Dan Huang
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China
Jian-Lin Jiang
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China
Xue-Mei Luo
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China
Jian-Hua Miao
Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China
Tian-Qi Liu
Jiangbin Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, China
Shuai Zhao
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China; Corresponding author.
Jia-Xun Feng
State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China; Corresponding author. College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, China.
Due to the scarcity of wild fruiting bodies, submerged fermentation of the medicinal fungus Antrodia camphorata is attracting much attention, but the production of bioactive triterpenoids is low. Therefore, there is an urgent need to improve the triterpenoid yield of submerged fermentation. Here, the A. camphorata mutant E3-64 was generated from strain AC16101 through random mutagenesis breeding, producing 172.8 mg triterpenoid per gram of dry mycelia. Further optimization of culture parameters resulted in a yield of 255.5 mg/g dry mycelia (i.e., an additional >1.4-fold increase), which is the highest reported yield thus far. Notably, mutant E3-64 produced 94% and 178% more of the triterpenoid components antcin A and antcamphin A, respectively, while it produced 52% and 15% less antcin B and G, respectively. Mutant E3-64 showed increased expression of key genes involved in triterpenoid biosynthesis, as well as different genome-wide single-nucleotide polymorphisms as compared with AC16101. Triterpenoids of the E3-64 mycelia exhibited remarkably protective activity against acute CCl4-induced liver injury in mice. This study shows the potential of A. camphorata for scientific research and commercial application.
