Nature Communications (Mar 2020)
Two distinct modes of DNMT1 recruitment ensure stable maintenance DNA methylation
- Atsuya Nishiyama,
- Christopher B. Mulholland,
- Sebastian Bultmann,
- Satomi Kori,
- Akinori Endo,
- Yasushi Saeki,
- Weihua Qin,
- Carina Trummer,
- Yoshie Chiba,
- Haruka Yokoyama,
- Soichiro Kumamoto,
- Toru Kawakami,
- Hironobu Hojo,
- Genta Nagae,
- Hiroyuki Aburatani,
- Keiji Tanaka,
- Kyohei Arita,
- Heinrich Leonhardt,
- Makoto Nakanishi
Affiliations
- Atsuya Nishiyama
- Division of Cancer Cell Biology, The Institute of Medical Science, The University of Tokyo
- Christopher B. Mulholland
- Department of Biology II and Center for Integrated Protein Science Munich (CIPSM), Human Biology and BioImaging, Ludwig-Maximilians-Universität München
- Sebastian Bultmann
- Department of Biology II and Center for Integrated Protein Science Munich (CIPSM), Human Biology and BioImaging, Ludwig-Maximilians-Universität München
- Satomi Kori
- Structure Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University
- Akinori Endo
- Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science
- Yasushi Saeki
- Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science
- Weihua Qin
- Department of Biology II and Center for Integrated Protein Science Munich (CIPSM), Human Biology and BioImaging, Ludwig-Maximilians-Universität München
- Carina Trummer
- Department of Biology II and Center for Integrated Protein Science Munich (CIPSM), Human Biology and BioImaging, Ludwig-Maximilians-Universität München
- Yoshie Chiba
- Division of Cancer Cell Biology, The Institute of Medical Science, The University of Tokyo
- Haruka Yokoyama
- Structure Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University
- Soichiro Kumamoto
- Division of Cancer Cell Biology, The Institute of Medical Science, The University of Tokyo
- Toru Kawakami
- Laboratory of Protein Organic Chemistry, Institute for Protein Research, Osaka University
- Hironobu Hojo
- Laboratory of Protein Organic Chemistry, Institute for Protein Research, Osaka University
- Genta Nagae
- The Research Center for Advanced Science and Technology, University of Tokyo
- Hiroyuki Aburatani
- The Research Center for Advanced Science and Technology, University of Tokyo
- Keiji Tanaka
- Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science
- Kyohei Arita
- Structure Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University
- Heinrich Leonhardt
- Department of Biology II and Center for Integrated Protein Science Munich (CIPSM), Human Biology and BioImaging, Ludwig-Maximilians-Universität München
- Makoto Nakanishi
- Division of Cancer Cell Biology, The Institute of Medical Science, The University of Tokyo
- DOI
- https://doi.org/10.1038/s41467-020-15006-4
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 17
Abstract
Ubiquitylation of histone H3 (H3Ub2) by UHRF1 recruits DNMT1 to chromatin, which is essential for DNA methylation inheritance. Here, the authors provide evidence that there are two distinct mechanisms underlying replication timing-dependent recruitment of DNMT1 through PAF15Ub2 and H3Ub2, both of which are required for high fidelity DNA methylation inheritance.
