Nature Communications (Mar 2021)

Single-cell measurement of plasmid copy number and promoter activity

  • Bin Shao,
  • Jayan Rammohan,
  • Daniel A. Anderson,
  • Nina Alperovich,
  • David Ross,
  • Christopher A. Voigt

DOI
https://doi.org/10.1038/s41467-021-21734-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 9

Abstract

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A quantitative assessment of promoter function can improve the precision of cellular engineering. Here the authors develop a method to simultaneously count plasmid DNA, RNA transcripts and protein expression in single living bacteria.