Single-cell measurement of plasmid copy number and promoter activity

Bin Shao; Jayan Rammohan; Daniel A. Anderson; Nina Alperovich; David Ross; Christopher A. Voigt

doi:10.1038/s41467-021-21734-y

Nature Communications (Mar 2021)

Single-cell measurement of plasmid copy number and promoter activity

Bin Shao,
Jayan Rammohan,
Daniel A. Anderson,
Nina Alperovich,
David Ross,
Christopher A. Voigt

Affiliations

Bin Shao: Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology
Jayan Rammohan: Biosystems and Biomaterials Division, Material Measurement Laboratory, National Institute of Standards and Technology
Daniel A. Anderson: Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology
Nina Alperovich: Biosystems and Biomaterials Division, Material Measurement Laboratory, National Institute of Standards and Technology
David Ross: Biosystems and Biomaterials Division, Material Measurement Laboratory, National Institute of Standards and Technology
Christopher A. Voigt: Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology

DOI: https://doi.org/10.1038/s41467-021-21734-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 9

Abstract

Read online

A quantitative assessment of promoter function can improve the precision of cellular engineering. Here the authors develop a method to simultaneously count plasmid DNA, RNA transcripts and protein expression in single living bacteria.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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