Radiosensitization effects and ROS generation by high Z metallic nanoparticles on human colon carcinoma cell (HCT116) irradiated under 150 MeV proton beam
Raizulnasuha Abdul Rashid,
Safri Zainal Abidin,
Muhammad Afiq Khairil Anuar,
Takahiro Tominaga,
Hiroaki Akasaka,
Ryohei Sasaki,
Katahira Kie,
Khairunisak Abdul Razak,
Binh. T.T. Pham,
Brian S. Hawkett,
Mary-Ann Carmichael,
Moshi Geso,
Wan Nordiana Rahman
Affiliations
Raizulnasuha Abdul Rashid
Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Safri Zainal Abidin
Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Muhammad Afiq Khairil Anuar
Department of Clinical Radiology, Hiroshima International University, Hiroshima 739-452, Japan
Takahiro Tominaga
Department of Clinical Radiology, Hiroshima International University, Hiroshima 739-452, Japan
Hiroaki Akasaka
Division of Radiation Oncology, Kobe University Graduate School of Medicine, Kobe, Japan
Ryohei Sasaki
Division of Radiation Oncology, Kobe University Graduate School of Medicine, Kobe, Japan
Katahira Kie
Hyogo Ion Beam Medical Centre, Hyogo 679-5165, Japan
Khairunisak Abdul Razak
School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia
Binh. T.T. Pham
Key Centre for Polymers and Colloids, School of Chemistry, The University of Sydney, NSW 2006, Australia
Brian S. Hawkett
Key Centre for Polymers and Colloids, School of Chemistry, The University of Sydney, NSW 2006, Australia
Mary-Ann Carmichael
Discipline of Medical Radiation, School of Health & Biomedical Sciences, RMIT University, Bundoora, Victoria, 3083 Australia
Moshi Geso
Discipline of Medical Radiation, School of Health & Biomedical Sciences, RMIT University, Bundoora, Victoria, 3083 Australia
Wan Nordiana Rahman
Medical Radiation Programme, School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; Corresponding author.
Radiosensitization effects caused by high atomic number (Z) metallic nanoparticles have been extensively explored with a wide range of X-ray based radiotherapy beams that seem to provide intriguing results for potential clinical applications. In this study, the radiobiological consequences of gold nanoparticles (AuNPs), superparamagnetic iron oxide nanoparticles (SPIONs), Platinum Nanodendrites (PtNDs) and Bismuth Oxide Nanorods (BiNRs) on Human Colon Carcinoma cells (HCT 116) irradiated with 150 MeV proton beams were investigated. Cell survival and reactive oxygen species (ROS) generation were measured. BiNRs demonstrates the highest sensitization enhancement ratio (SER) of 4.93 followed by PtNDs with SER value of 3.08. Meanwhile, AuNPs and SPIONs depict SER values around 2.64 and 1.95 respectively. The ROS generation corresponded to the level of radiosensitization with the highest ROS obtained for BiNRs and followed by PtNDs, AuNPs and SPIONs. In conclusion, high Z nanoparticles possess the potential to be clinically applied in proton beam therapy. Keywords: Gold nanoparticles, Superparamagnetic iron oxide nanoparticles, Platinum nanoparticles, Bismuth nanoparticles, Radiosensitization, Proton beam
