Armaghane Danesh Bimonthly Journal (Apr 2018)
Study mortality effects of carob (ceratonia silique) extract on protoscoleces of hydratid cyst in vitro
Abstract
Background and aim: The use of Chemotherapy for the treatment of hydatid cysts can be problematic due to different adverse effects. As a result, there is an increasing interest in non-chemical alternatives, such as medicinal plant extracts as new therapeutic tools against hydatid cyst. In the present study, various concentrations of carob extract at different exposure times were evaluated under laboratory conditions for their scolicidal effects on Hydatid Cysts. Methods: Protoscolices were aseptically collected from sheep livers having hydatid cysts and were exposed to three different concentrations of oak and carob extract (10, 25 and 50) mg /ml for 10, 20, 30 and 60 min. Viability of protoscolices was determined by 0.1% eosin staining. Results: Based on our results, the scolicidal effects of this plant on Hydatid Cysts in all concentrations was significant compared to the control groups (p<0.05). Carob extract at concentration of 50 mg/ml destroyed all protoscolices in 30 min. Conclusion: The results also showed that the extract of this plant produce high scolicidal activity; However, in vivo efficacy of this medicinal plant remains to be studied for treatment of hydatid cysts in humans and herbivorous animals.
