OncoTargets and Therapy (Dec 2019)
LINC00096 Promotes the Proliferation and Invasion by Sponging miR-383-5p and Regulating RBM3 Expression in Triple-Negative Breast Cancer
Abstract
Yanyan Tian, Shuguan Xia, Mingde Ma, Yonggang Zuo Department of Thyroid and Breast Surgery, Huaihe Hospital of Henan University, Kaifeng 475000, People’s Republic of ChinaCorrespondence: Yanyan TianDepartment of Thyroid and Breast Surgery, Huaihe Hospital of Henan University, No.8 Baobei Road, Gulou District, Kaifeng 475000, People’s Republic of ChinaEmail [email protected]: Recent studies revealed that long non-coding RNAs (lncRNA) play crucial roles in cancer initiation and progression. However, the function and underlying mechanism of lncRNAs in triple-negative breast cancer (TNBC) are little investigated.Methods: qRT-PCR was used to investigate LINC00096 expression in TNBC tissues and cells. Function assays were used to test the effects of LINC00096 on TNBC cells progression. In addition, luciferase reporter and qRT-PCR assays were used to determine the underlying mechanism of LINC00096 on TNBC progression.Results: In our present study, we identify LINC00096 as one of the most upregulated lncRNA in TNBC progression by using microarray screening. High LINC00096 expression was obviously related to advanced tumor stage, metastasis, poor prognosis of patients. Loss-of-function assays showed that LINC00096 suppression reduced TNBC cells proliferation and invasive abilities in vitro. Mechanistically, we demonstrated that LINC00096 directly interacted with miR-383-5p, subsequently acted as a miRNA sponge to increase RBM3 expression.Conclusion: In the present study, we indicated that LINC00096 might promote the proliferation and invasion through regulating the miR-383-5p/RBM3 pathway in TNBC, which providing a novel therapeutic target for cancer treatment.Keywords: LINC00096, miR-383-5p, RBM3, triple-negative breast cancer
