In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida Albicans

Abstract

The aim of this in vitro study was to evaluate the minimal inhibitory concentration (MIC) of 3% NaOCl, 2% CHX, MTAD, and EDTA against Candida albicans (C. albicans). Methods and Results: Certified strain of C. albicans (ATCC 10231 OXOID, Hampshire, UK) was used to determine the MIC of 3% NaOCl, 2% CHX, MTAD, and EDTA in vitro. The broth dilution method was used to determine the MIC and to ensure the test was highly accurate. C. albicans and respective irrigants were gradually placed into the appropriate test tubes, starting from 1 mL to 0.06 mL of irrigant (getting halved each time). The test tubes were incubated at 37°C for 24 h. MIC was then recorded as the lowest concentration of irrigant that inhibited microbial growth, based on based on spectrophotometry. It was found the higher the value of the absorbance rate, the smaller the antifungal effectiveness of the tested substance. Our study showed that some of the tested irrigants retained an antifungal effect after dilution, which is valuable because dilution reduces toxicity. 3% NaOCl has an efficient antifungal effect against C. albicans both at full concentration and when diluted fivefold. The antifungal effect of 2% CHX for C. albicans cultures increases with its dilution. MTAD retains a good antifungal effect even when diluted fivefold.

Keywords

• candida albicans
• minimal inhibitory concentration
• mtad
• chx
• naocl
• edta