Zhongguo youzhi (Jan 2024)

黄曲霉毒素B1 降解菌株的分离鉴定、发酵条件的 优化及活性组分分析Isolation and identification of aflatoxin B1-degrading strains, optimization of fermentation conditions and analysis of active components

  • 陈毅保，刘昆仑，杨趁仙，李艳，李天赐，贾叶萍，张微漾 CHEN Yibao，LIU Kunlun，YANG Chenxian，LI Yan，LI Tianci， JIA Yeping，ZHANG Weiyang

DOI
https://doi.org/10.19902/j.cnki.zgyz.1003-7969.220714
Journal volume & issue
Vol. 49, no. 1
pp. 120 – 126

Abstract

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为筛选出一株高效降解黄曲霉毒素B1（AFB1）的菌株，选取土壤为菌株来源，进行富集培养，经过以香豆素为唯一碳源的初筛和添加AFB1的复筛后，对筛选得到的各菌株进行16S rDNA鉴定，比对其测序结果，选取降解AFB1最高的菌株，探究发酵时间、发酵温度、初始pH、接种量、培养基对菌株降解AFB1的影响规律，通过正交试验优化发酵条件，并对降解方式进行初步探索。结果表明：经过初筛和复筛得到7株能够降解AFB1的菌株，经鉴定均为伯克霍尔德菌属，其中Burkholderia sp. D6的AFB1降解率最高；最优发酵条件为以LB培养基为发酵培养基、发酵时间84 h、发酵温度37 ℃、初始pH 7.0、接种量10%，在此条件下Burkholderia sp. D6对AFB1的降解率为（87.91±2.32)%；初步判断降解AFB1的活性组分是蛋白质或者酶。综上，通过筛选得到了一种能够高效降解AFB1的菌株，蛋白质或酶参与了AFB1的降解。In order to screen a strain that can efficiently degrade aflatoxin B1（AFB1）, soil was selected as the source of screening bacteria for enrichment culture. After preliminary screening with coumarin as the only carbon source and rescreening with AFB1 added, and 16S rDNA identification was performed on the selected strains, and their sequencing results were compared. The strain with the highest degradation rate of AFB1 was selected, the effects of fermentation time, fermentation temperature, initial pH, inoculation amount, and medium on the degradation rate of AFB1 were studied, the fermentation conditions were optimized by orthogonal experiment, and the degradation method was preliminarily explored. The results showed that 7 strains capable of degrading AFB1 were screened by preliminary screening and rescreening, which were indentified as Burkholderia sp. , and Burkholderia sp.D6 had the highest degradation rate of AFB1. The optimal fermentation conditions were obtained as follows: LB fermentation medium, fermentation time 84 h, fermentation temperature 37 ℃, initial pH 7.0, inoculation amount 10%. Under these conditions, the degradation rate of AFB1 was (87.91±2.32)%. It was preliminarily judged that the active substance for degrading AFB1 was protein or enzyme. In conclusion, a strain capable of efficiently degrading AFB1 is screened, and protein or enzyme are involved in the degradation of AFB1.

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