Quantitative Detection of Living Yeast Fraction From Mixed Living and Dead Cell Solution by Micro Electrical Impedance Spectroscopy

Xiayi Liu; Yahui Cui; Tong Zhao; Daisuke Kawashima; Hiromichi Obara; Masahiro Takei

doi:10.1109/ACCESS.2019.2902576

IEEE Access (Jan 2019)

Quantitative Detection of Living Yeast Fraction From Mixed Living and Dead Cell Solution by Micro Electrical Impedance Spectroscopy

Xiayi Liu,
Yahui Cui,
Tong Zhao,
Daisuke Kawashima,
Hiromichi Obara,
Masahiro Takei

Affiliations

Xiayi Liu: Faculty of Mechanical and Precision Instrument Engineering, Xi’an University of Technology, Xi’an, China
Yahui Cui: Faculty of Mechanical and Precision Instrument Engineering, Xi’an University of Technology, Xi’an, China
Tong Zhao: ORCiD; Faculty of Mechanical and Precision Instrument Engineering, Xi’an University of Technology, Xi’an, China
Daisuke Kawashima: Department of Mechanical Engineering, Chiba University, Chiba, Japan
Hiromichi Obara: Department of Mechanical Engineering, Tokyo Metropolitan University, Tokyo, Japan
Masahiro Takei: Department of Mechanical Engineering, Chiba University, Chiba, Japan

DOI: https://doi.org/10.1109/ACCESS.2019.2902576
Journal volume & issue: Vol. 7
pp. 33970 – 33977

Abstract

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An empirical model is proposed to detect the living cell fraction Φ in a multi-mixed cell solution by micro electrical impedance spectroscopy (EIS) as Φ = -1.46/ψ0 + 1.97. The living cell indicator ψ0 is defined by a quotient of the extrema in the impedance imaginary parts of the detected cell solution's medium, Zextmed, and referenced Zextmedref. The theoretical effectiveness of the model is examined by an electrochemical simulation. In addition, the sensor size versatility is discussed by a comparison experiment that reveals that the sensor size has little effect on the detection result. To support real-time detection, Zextmed and Zextmedref are replaced by the impedance of the cell solution, Zextm, and Zextmref. The versatility of real-time detection is discussed by a medium similarity coefficient η( f) that is defined to explain the effect of replacement. The results reveal the feasibility of the replacement as η > 95% in the injected current frequency range of ψ0 by micro EIS.

Published in IEEE Access

ISSN: 2169-3536 (Online)
Publisher: IEEE
Country of publisher: United States
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering
Website: https://ieeexplore.ieee.org/xpl/RecentIssue.jsp?punumber=6287639

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