Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA

Howard Gamper; Haixing Li; Isao Masuda; D. Miklos Robkis; Thomas Christian; Adam B. Conn; Gregor Blaha; E. James Petersson; Ruben L. Gonzalez; Ya-Ming Hou

doi:10.1038/s41467-020-20373-z

Nature Communications (Jan 2021)

Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA

Howard Gamper,
Haixing Li,
Isao Masuda,
D. Miklos Robkis,
Thomas Christian,
Adam B. Conn,
Gregor Blaha,
E. James Petersson,
Ruben L. Gonzalez,
Ya-Ming Hou

Affiliations

Howard Gamper: Department of Biochemistry and Molecular Biology, Thomas Jefferson University
Haixing Li: Department of Chemistry, Columbia University
Isao Masuda: Department of Biochemistry and Molecular Biology, Thomas Jefferson University
D. Miklos Robkis: Department of Chemistry, University of Pennsylvania
Thomas Christian: Department of Biochemistry and Molecular Biology, Thomas Jefferson University
Adam B. Conn: Department of Biochemistry, University of California
Gregor Blaha: Department of Biochemistry, University of California
E. James Petersson: Department of Chemistry, University of Pennsylvania
Ruben L. Gonzalez: Department of Chemistry, Columbia University
Ya-Ming Hou: Department of Biochemistry and Molecular Biology, Thomas Jefferson University

DOI: https://doi.org/10.1038/s41467-020-20373-z
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 18

Abstract

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Genome recoding with quadruplet codons requires a +1-frameshift-suppressor tRNA able to insert an amino acid at quadruplet codons of interest. Here the authors identify the mechanisms resulting in +1 frameshifting and the steps of the elongation cycle in which it occurs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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