Collection and Identification of Native Rhizobium Strains and Assessment of their Symbiosis with Progressive Persian Clover Lines

Abstract

In order to introducing efficient ecological genotype and native rhizobium strains in persian clover (Trifolium resupinatum L.) this study was conducted during 2003-2011. Since 2003, native persian clover populations were collected in Lorestan province, Iran, and after different studies15 promising lines were selected.Then, 15 native Rhizobium legominosarum bv. trifollii strains were collected and identified from the same area. In 2010, a greenhouse factorial experiment based on randomized complete design in three replications was conducted with 240 treatments (15 line × 16 strain) in research station of Borujerd. In this study symbiosis index, root and above-ground dry matters, root:shoot proportion, stem height, nodule number and nitrogen content of plant tissue were assessed. Grouping lines and strains was performed by cluster analysis under Ward method. Results showed that interaction of line × strain were significant (p

Keywords

• Ecological Genotype
• Native Rhizobium
• Nitrogen
• Persian Clover
• Symbiosis