SENSIBILITY OF MICROCULTIVATION ASSAY DERMATOBAC® FOR FUNGAL ENDOMETRITIS DIAGNOSIS IN MARES

Abstract

Among different etiologic factors, fungal endometritis is a common cause of decreased mare’s fertility. Two experiments were conducted to evaluate the sensitivity of Dermatobac®, a microculture system originallydevelopedfortheisolationoffungithatconcerns Human Medicine. In Experiment 1, standard (ATCC) strains of Candida (n=4) and Aspergyllus (n=1) were grown and visually analyzed daily for 7 days. For Experiment 2, 34 mares were selected and subjected to material harvesting for completion of uterine cytology as well as seeding with Dermatobac®. After 24h of cultivation, the macroscopic observation of all Candida and Aspergyllus strains (Experiment 1) was possible. In Experiment 2, five animals showed presence of yeast on cytologic slides (14.71%), results also confirmed sowing in Dermatobac® system (100% sensitivity). Five false positive cases were observed, characterized by microbiological growth in Dermatobac® but not confirmed by endometrial cytology, determining specificity of 85%. However, the results did not compromise the efficiency of Dermatobac® system given all contaminants presented differentiated morphology and higher growing time (P<0.05) when compared to the major etiological agents of equine fungal endometritis. The Dermatobac® system proved to be efficient for the macroscopic diagnosis of fungal endometritis in horses, showing conclusive information only after 24 hours of culture.

Keywords

• Dermatobac®
• diagnosis
• endometritis
• fungi; mare.