Quantitative Trait Loci Mapping and Association Analysis of Solanesol Content in Tobacco (<i>Nicotiana tabacum</i> L.)
Jing Liu,
Dehu Xiang,
Yongmei Du,
Zhongfeng Zhang,
Hongbo Zhang,
Lirui Cheng,
Qiujuan Fu,
Ning Yan,
Fuzhu Ju,
Chaofan Qi,
Yunkang Lei,
Jun Wang,
Yanhua Liu
Affiliations
Jing Liu
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Dehu Xiang
Shimen County Branch of Changde Company of Hunan Tobacco Company, Changde 415300, China
Yongmei Du
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Zhongfeng Zhang
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Hongbo Zhang
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Lirui Cheng
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Qiujuan Fu
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Ning Yan
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Fuzhu Ju
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Chaofan Qi
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Yunkang Lei
Sichuan Tobacco Corporation Deyang Branch, Deyang 618400, China
Jun Wang
Tobacco Science Institute of Guangdong Province, Shaoguan 512026, China
Yanhua Liu
Plant Functional Ingredient Research Center, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, 11 Keyuanjingsi Road, Qingdao 266101, China
Solanesol, which accumulates predominantly in the leaves of tobacco plants, has medically important bioactive properties. To investigate the genetic basis of solanesol in tobacco (Nicotiana tabacum), the solanesol contents of 222 accessions, 206 individuals from an N. tabacum Maryland609 (low-solanesol) × K326 (high-solanesol) F2 population and their corresponding F1 self-pollinations, were determined using ultra-performance liquid chromatography. Genome-wide quantitative trait locus (QTL) and association analysis were performed to identify QTLs and markers associated with solanesol content based on simple sequence repeat molecular markers. A total of 12 QTLs underlying solanesol content were mapped to seven linkage groups (LGs), with three of the QTLs (QTL3-1, QTL21-6, and QTL23-3) explaining 5.19–10.05% of the phenotypic variation. Association analysis revealed 38 significant marker-trait associations in at least one environment. The associations confirmed the QTLs located on LG3, LG10, LG14, LG21, and LG23, while new elite makers were located on 11 additional LGs, each explaining, respectively, 5.16–20.07% of the phenotypic variation. The markers LG14-PT54448, LG10-PT60114-2, LG10-PT60510, LG10-PT61061, and LG-21PT20388 may be useful for molecular-assisted selection of solanesol content in tobacco leaves. These results increase our understanding of the inheritance of solanesol-associated genes and will contribute to molecular-assisted breeding and further isolation of regulatory genes involved in solanesol biosynthesis in tobacco leaves.
