Fructo-Oligosaccharide (DFA III) Feed Supplementation for Mitigation of Mycotoxin Exposure in Cattle—Clinical Evaluation by a Urinary Zearalenone Monitoring System
Katsuki Toda,
Seiichi Uno,
Emiko Kokushi,
Ayaka Shiiba,
Hiroshi Hasunuma,
Daisaku Matsumoto,
Masayuki Ohtani,
Osamu Yamato,
Urara Shinya,
Missaka Wijayagunawardane,
Johanna Fink-Gremmels,
Masayasu Taniguchi,
Mitsuhiro Takagi
Affiliations
Katsuki Toda
United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan
Seiichi Uno
Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan
Emiko Kokushi
Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, Japan
Ayaka Shiiba
United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan
Hiroshi Hasunuma
Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan
Daisaku Matsumoto
Shepherd Central Livestock Clinic, Kagoshima 899-1611, Japan
Masayuki Ohtani
Nippon Beet Sugar Manufacturing Co., Ltd., Obihiro 080-0835, Japan
Osamu Yamato
Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0062, Japan
Urara Shinya
Soo Agricultural Mutual Aid Association, Kagoshima 890-8212, Japan
Missaka Wijayagunawardane
Department of Animal Science, University of Peradeniya, Peradeniya 20400, Sri Lanka
Johanna Fink-Gremmels
Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, The Netherlands
Masayasu Taniguchi
United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan
Mitsuhiro Takagi
United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan
The potential effect of difructose anhydride III (DFA III) supplementation in cattle feed was evaluated using a previously developed urinary-zearalenone (ZEN) monitoring system. Japanese Black cattle from two beef herds aged 9–10 months were used. DFA III was supplemented for two weeks. ZEN concentrations in feed were similar in both herds (0.27 and 0.22 mg/kg in roughage and concentrates, respectively), and below the maximum allowance in Japan. ZEN, α-zearalenol (α-ZOL), and β-ZOL concentrations in urine were measured using LC/MS/MS the day before DFA III administration, 9 and 14 days thereafter, and 9 days after supplementation ceased. Significant differences in ZEN, α-ZOL, β-ZOL, and total ZEN were recorded on different sampling dates. The concentration of inorganic phosphate in DFA III-supplemented animals was significantly higher than in controls on day 23 (8.4 vs. 7.7 mg/dL), suggesting a possible role of DFA III in tight junction of intestinal epithelial cells. This is the first evidence that DFA III reduces mycotoxin levels reaching the systemic circulation and excreted in urine. This preventive effect may involve an improved tight-junction-dependent intestinal barrier function. Additionally, our practical approach confirmed that monitoring of urinary mycotoxin is useful for evaluating the effects of dietary supplements to prevent mycotoxin adsorption.
