Defining the ATPome reveals cross-optimization of metabolic pathways

Neal K. Bennett; Mai K. Nguyen; Maxwell A. Darch; Hiroki J. Nakaoka; Derek Cousineau; Johanna ten Hoeve; Thomas G. Graeber; Markus Schuelke; Emin Maltepe; Martin Kampmann; Bryce A. Mendelsohn; Jean L. Nakamura; Ken Nakamura

doi:10.1038/s41467-020-18084-6

Nature Communications (Aug 2020)

Defining the ATPome reveals cross-optimization of metabolic pathways

Neal K. Bennett,
Mai K. Nguyen,
Maxwell A. Darch,
Hiroki J. Nakaoka,
Derek Cousineau,
Johanna ten Hoeve,
Thomas G. Graeber,
Markus Schuelke,
Emin Maltepe,
Martin Kampmann,
Bryce A. Mendelsohn,
Jean L. Nakamura,
Ken Nakamura

Affiliations

Neal K. Bennett: Gladstone Institute of Neurological Disease
Mai K. Nguyen: Gladstone Institute of Neurological Disease
Maxwell A. Darch: Gladstone Institute of Neurological Disease
Hiroki J. Nakaoka: Department of Radiation Oncology, University of California
Derek Cousineau: Gladstone Institute of Neurological Disease
Johanna ten Hoeve: UCLA Metabolomics Center, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California
Thomas G. Graeber: UCLA Metabolomics Center, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California
Markus Schuelke: NeuroCure Clinical Research Center, Charité–Universitätsmedizin Berlin
Emin Maltepe: Department of Pediatrics, University of California
Martin Kampmann: Department of Biochemistry and Biophysics and Institute for Neurodegenerative Diseases, University of California
Bryce A. Mendelsohn: Gladstone Institute of Neurological Disease
Jean L. Nakamura: Department of Radiation Oncology, University of California
Ken Nakamura: Gladstone Institute of Neurological Disease

DOI: https://doi.org/10.1038/s41467-020-18084-6
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 16

Abstract

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Energy metabolism and ATP levels are controlled by an interlocking network of pathways. Here, the authors apply a genome-wide CRISPR screen to define genes that increase or decrease ATP levels to define the “ATPome”, a map of pathways that contribute to cellular ATP regulation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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