Journal of Biomedical Photonics & Engineering (May 2017)

Analysis of albumin Raman scattering registration efficiency from different volume and shape cuvette

  • Anastasia A. Lykina,
  • Dmitry N. Artemyev,
  • Ivan A. Bratchenko

DOI
https://doi.org/10.18287/JBPE17.03.020309
Journal volume & issue
Vol. 3, no. 2

Abstract

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The cuvette shape influence on the Raman registration efficiency at small volumes of albumin (0.15-1 ml) using the PLS model was analysed. This paper examines the smallest measurement error RMSE of 3-6 g/l with a correlation coefficient R2 = 0.95 was achieved for pure Raman spectra extraction by baseline correction with asymmetric least squares smoothing method for aluminum spherical type cuvette-reflectors. Its curvature center was matched with the focal spot of the exciting laser. The error of albumin concentration determination equaled about 5% of albumin normal concentration in a blood plasma using a spherical cuvette with a volume of 0.55 ml. A further decrease of the cuvette volume led to a sharp loss of the method prediction accuracy.

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