Generation of integration-free induced pluripotent stem cells from a patient with spina bifida

Hongran Wang; Shuying Zhao; Richard H. Finnell; Timothy George; Austin J. Cooney

Stem Cell Research (Aug 2018)

Generation of integration-free induced pluripotent stem cells from a patient with spina bifida

Hongran Wang,
Shuying Zhao,
Richard H. Finnell,
Timothy George,
Austin J. Cooney

Affiliations

Hongran Wang: Department of Pediatrics, Dell Pediatric Research Institute, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States
Shuying Zhao: Department of Surgery and Perioperative Care, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States
Richard H. Finnell: Department of Pediatrics, Dell Pediatric Research Institute, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States
Timothy George: Department of Pediatrics, Dell Pediatric Research Institute, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States; Department of Surgery and Perioperative Care, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States
Austin J. Cooney: Department of Pediatrics, Dell Pediatric Research Institute, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States; Corresponding author.

Journal volume & issue: Vol. 31
pp. 27 – 30

Abstract

Read online

A skin biopsy was obtained from a 14-year-old female patient with a history of Myelomeningocele. Dermal fibroblasts were isolated and reprogrammed with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. The generated induced Pluripotent Stem Cell (iPSC) clones NTDi4_09A were free of genomically integrated reprogramming genes, had a stable normal karyotype and expressed pluripotency markers. The iPSCs formed teratomas in mice, which were differentiated towards derivatives of the three germ layers in vivo. This iPSC line offers a useful resource to study a genetic profile of a patient with spina bifida.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal