study the effect of alcoholic and watery extracts of garlic and onion plants on growth of candida albicans and Cryptococcus neoformans in vitro&vivo

Abstract

This research included study the effect of garlic and onion plants extracts(alcoholic and watery) in vitro in three different concentrations 15%,25%,35% and in vivo in experimental white mice .Research was performed by three experiments, first one was conducted to study effectiveness of different concentration of alcoholic and watery garlic extract on growth of candida albicans and Cryptococcus neoformans in vitro, showed that the effect of alcoholic extract on the growth of candida albicans was inhibitory,started from 0.4 mm to 0.1 mm compared with control plats 4.2 mm ,where as the results of the effect on the growth of Cryptococcus neoformans showed more clearness and the inhibition started from 0.6 to inhibit all the growth in plat in comparison with control plats1.4 mm. While the effect of watery garlic extract showed less effect and the inhibition began from 0.5 mm to 0.2 mm for candida albicans , but the growth inhibition of Cryptococcus neoformans began from 0.4 to 0.15 mm.The second experiment was the same as the first experiment , but using alcoholic and extracts onion , the growth of candida albicans inhibited by alcoholic exract from 0.6 mm to no growth in the plat , but the inhibition of Cryptococcus neoformans was started from 0.5mm to 0.2 mm for alcoholic onion extract. While the watery onion extract effect on the growth of candida albicans the inhibition started from 1.6 mm to 1 mm ,but the inhibition of Cryptococcus neoformans was began from 1 mm to 0.3 mm. Third experiment was study the effect of crude garlic and onion alcoholic extract ointment 1% on experimental infection in mice , using 30 experimental mice divided to 6 equal groups,each group include 5 mice *groups which infected with candida albicans treated : The group 1,2,3,expermrutly infected with candida albicans ,where as group 3,4,6 were infected with Cryptococcus neoformans for 1,2,3 group ,treted with the ointment of alcoholic extract of garlic, group 2 treated with alcoholic extract ointment of onion, where as group 3 left with no treatment as a control group