New Detection Method for Fungal Infection in Silver Fir Seeds

Abstract

Silver fir trees have cycles of low and high seed production, and thus it is necessary to collect seeds in high production years to save them for low production years to ensure the continuity of nursery production. Tree seeds can be stored loosely in piles or containers, but they need to be checked for viability before planting. The objective of this study was to find a quick and inexpensive method to determine the suitability of seed lots for planting. The working hypothesis was that an electronic nose device could be used to detect odors from fungi or from decomposing organic material, and thus aid in determination of whether seeds could be sown or discarded. To affirm and supplement results from the electronic nose, we used gas chromatography–mass spectrometry (GC-MS) to detect volatile secondary metabolites such as limonene and cadienes, which were found at the highest concentrations in both, infected and uninfected seeds. Uninfected seeds contained exceptionally high concentrations of pinene, which are known to be involved in plant resistance responses. Statistically higher levels of terpineol were found in infected seeds than in uninfected seeds. A prototype of our electronic nose partially discriminated between healthy and spoiled seeds, and between green and white fungal colonies grown on incubated seeds. These preliminary observations were encouraging and we plan to develop a practical device that will be useful for forestry and horticulture.

Keywords

• seeds
• silver fir
• <i>Abies alba</i> Mill.
• GC-MS
• secondary metabolites
• electronic nose