Nutrient Media for Diagnosis of Brucellosis

Abstract

The overview contains information from Russian and foreign literature about composition and usage of nutrient media for brucellosis diagnosis and immunobiological preparations production. Nutrient media for cultivation, isolation, enrichment, identification and differentiation of Brucella and their L-form as well as transport media and synthetic media are considered. Advantages and disadvantages are described for some of the products. The overview also contains information about nutritional bases, growth factors and selective components included in these media. There is a particular focus on selective media, their history and improvement. The article gives the requirements for the inhibitory properties of these products and the disadvantages of using certain antimicrobial agents. The authors list nutrient media for various purposes, certified for use by the Russian regulatory documents, recommendations of World Health Organization and World Organization for Animal Health. Data about principles for assessing the quality of culture media and test strains used to control biological parameters of culture media are covered. The authors also present materials about the trends in nutrient media development for Brucella cultivation and isolation, methods of their application. In conclusion, they identify the need to conduct research in the sphere of the development of new nutrient media for Brucella isolation from the material contaminated by foreign microorganisms.

Keywords

• <i>brucella</i>
• nutrient media
• cultivation
• isolation
• identification
• differentiation
• test strain