Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study

A. Marmotti; S. Mattia; M. Bruzzone; S. Buttiglieri; A. Risso; D. E. Bonasia; D. Blonna; F. Castoldi; R. Rossi; C. Zanini; E. Ercole; E. Defabiani; C. Tarella; G. M. Peretti

doi:10.1155/2012/326813

Stem Cells International (Jan 2012)

Minced Umbilical Cord Fragments as a Source of Cells for Orthopaedic Tissue Engineering: An In Vitro Study

A. Marmotti,
S. Mattia,
M. Bruzzone,
S. Buttiglieri,
A. Risso,
D. E. Bonasia,
D. Blonna,
F. Castoldi,
R. Rossi,
C. Zanini,
E. Ercole,
E. Defabiani,
C. Tarella,
G. M. Peretti

Affiliations

A. Marmotti: Department of Orthopaedics and Traumatology, University of Turin, Mauriziano Hospital, Largo Turati 62, 10128 Turin, Italy
S. Mattia: Molecular Biotechnology Center, University of Turin, Via Nizza 52, 10126 Turin, Italy
M. Bruzzone: Department of Orthopaedics and Traumatology, University of Turin, Mauriziano Hospital, Largo Turati 62, 10128 Turin, Italy
S. Buttiglieri: Molecular Biotechnology Center, University of Turin, Via Nizza 52, 10126 Turin, Italy
A. Risso: Molecular Biotechnology Center, University of Turin, Via Nizza 52, 10126 Turin, Italy
D. E. Bonasia: Department of Orthopaedics and Traumatology, University of Turin, Mauriziano Hospital, Largo Turati 62, 10128 Turin, Italy
D. Blonna: Department of Orthopaedics and Traumatology, University of Turin, Mauriziano Hospital, Largo Turati 62, 10128 Turin, Italy
F. Castoldi: Department of Orthopaedics and Traumatology, University of Turin, Mauriziano Hospital, Largo Turati 62, 10128 Turin, Italy
R. Rossi: Department of Orthopaedics and Traumatology, University of Turin, Mauriziano Hospital, Largo Turati 62, 10128 Turin, Italy
C. Zanini: Research & Development Department, EuroClone S.p.A., Molecular Biotechnology Center, University of Turin, Via Nizza 52, 10126 Turin, Italy
E. Ercole: Research & Development Department, EuroClone S.p.A., Molecular Biotechnology Center, University of Turin, Via Nizza 52, 10126 Turin, Italy
E. Defabiani: Department of Obstetrics and Gynaecology, University of Turin, Mauriziano Hospital, Largo Turati 62, 10128 Turin, Italy
C. Tarella: Molecular Biotechnology Center, University of Turin, Via Nizza 52, 10126 Turin, Italy
G. M. Peretti: Department of Sport, Nutrition and Health Sciences, University of Milan, Via Kramer 4/A, 20129 Milan, Italy

DOI: https://doi.org/10.1155/2012/326813
Journal volume & issue: Vol. 2012

Abstract

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A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of 4,2×106 cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.

Published in Stem Cells International

ISSN: 1687-966X (Print); 1687-9678 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine
Website: https://onlinelibrary.wiley.com/journal/4162

About the journal