Nature Communications (Feb 2022)

In vivo CRISPR screens reveal a HIF-1α-mTOR-network regulates T follicular helper versus Th1 cells

  • Bonnie Huang,
  • James D. Phelan,
  • Silvia Preite,
  • Julio Gomez-Rodriguez,
  • Kristoffer H. Johansen,
  • Hirofumi Shibata,
  • Arthur L. Shaffer,
  • Qin Xu,
  • Brendan Jeffrey,
  • Martha Kirby,
  • Stacie Anderson,
  • Yandan Yang,
  • Selamawit Gossa,
  • Dorian B. McGavern,
  • Louis M. Staudt,
  • Pamela L. Schwartzberg

DOI
https://doi.org/10.1038/s41467-022-28378-6
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 16

Abstract

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T follicular helper (Tfh) and T help type 1 (Th1) cells both arise from naïve CD4 T cells, but detailed knowledge of their differentiation remains incomplete. Here the authors pursue an in vivo CRISPR screen to identify genes, focusing on druggable targets, regulating Tfh versus Th1 to provide a resource for related studies, while also implicating HIF-1α and VHL in this regulation.