Liposomes Loaded with Amaranth Unsaponifiable Matter and Soybean Lunasin Prevented Melanoma Tumor Development Overexpressing Caspase-3 in an In Vivo Model
Erick Damian Castañeda-Reyes,
María de Jesús Perea-Flores,
Gloria Dávila-Ortiz,
Elvira Gonzalez de Mejia
Affiliations
Erick Damian Castañeda-Reyes
Vegetal Proteins Laboratoy, Department of Biochemical Engineering, National School of Biological Sciences, National Polytechnic Institute, (IPN), Av. Wilfrido Massieu, and Miguel Stampa s/n, Zacatenco, Gustavo A. Madero, Mexico City 07738, Mexico
María de Jesús Perea-Flores
National Center of Nanosciences and Micro and Nanotechnologies, National Polytechnic Institute (IPN), Av. Luis Enrique Erro s/n, Unidad Profesional Adolfo López Mateos, Zacatenco, Alcaldía Gustavo A. Madero, Mexico City 07738, Mexico
Gloria Dávila-Ortiz
Vegetal Proteins Laboratoy, Department of Biochemical Engineering, National School of Biological Sciences, National Polytechnic Institute, (IPN), Av. Wilfrido Massieu, and Miguel Stampa s/n, Zacatenco, Gustavo A. Madero, Mexico City 07738, Mexico
Elvira Gonzalez de Mejia
Department of Food Science and Human Nutrition, 228 ER Madigan Laboratory, University of Illinois, 2101 W. Gregory Dr, Champaign, IL 61801, USA
The objective of this study was to assess the effectiveness of liposomes loaded with soybean lunasin and amaranth unsaponifiable matter (UM + LunLip) as a source of squalene in the prevention of melanoma skin cancer in an allograft mice model. Tumors were induced by transplanting melanoma B16-F10 cells into the mice. The most effective treatments were those including UM + LunLip, with no difference between the lunasin concentrations (15 or 30 mg/kg body weight); however, these treatments were statistically different from the tumor-bearing untreated control (G3) (p p p p < 0.0001). In conclusion, the UM + LunLip treatment was effective when applied either subcutaneously or topically in the melanoma tumor-developing groups, as it slowed down cell proliferation and activated apoptosis.
