Nature Communications (Nov 2021)

Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics

  • Jonathan Schubert,
  • Andrea Schulze,
  • Chrisostomos Prodromou,
  • Hannes Neuweiler

DOI
https://doi.org/10.1038/s41467-021-27286-5
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 12

Abstract

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Revealing mechanisms of complex protein machines requires simultaneous exploration of multiple structural coordinates. Here the authors report two-colour fluorescence microscopy combined with photoinduced electron transfer probes to simultaneously detect two structural coordinates in single protein molecules.