Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?
Dylan Henderson,
Christian Huebner,
Moses Markowitz,
Nicole Taube,
Zachary M. Harvanek,
Ursula Jakob,
Daniela Knoefler
Affiliations
Dylan Henderson
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Christian Huebner
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Moses Markowitz
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Nicole Taube
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Zachary M. Harvanek
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Ursula Jakob
Correspondence to: Department of Molecular, Cellular, and Developmental Biology, Natural Science Building, Room 4041, 830 N. University, Ann Arbor, MI 48109, USA.; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Daniela Knoefler
Correspondence to: Department of Molecular, Cellular, and Developmental Biology, Natural Science Building, Room 4041, 830 N. University, Ann Arbor, MI 48109, USA.; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Lifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans lifespan. We found that worms exposed to 25 °C during development and shifted to 15 °C in adulthood exhibited an even longer lifespan than animals constantly kept at 15 °C. Analysis of the in vivo redox status demonstrated that at 25 °C, C. elegans larvae have a more reduced redox state and higher Prdx-2 expression levels than animals raised at 15 °C. Worms lacking prdx-2 fail to show the additional lifespan extension upon shift from 25 °C to 15 °C and reveal a lifespan similar to prdx-2 worms always kept at 15 °C. These results suggest that transiently altering the in vivo redox state during development can have highly beneficial long-term consequences for organisms. Keywords: Aging, Temperature, C. elegans, Oxidants, Peroxiredoxin
