Preparation and photodynamic performance of electrospinning CA/PpIX porous fibrous membrane

Abstract

To address the problem of drug-resistant bacterial infections, a photodynamic broad-spectrum antibacterial cellulose acetate/protoporphyrin (CA/PpIX) porous microfibre membrane was prepared by electrospinning. The surface morphology of the fibrous membrane was observed by scanning electron microscope (SEM) and the distribution of PpIX on CA membrane was observed by laser confocal scanning microscope (CLSM). The change of the basic chemical structure of membrane before and after loading PpIX was analyzed by Fourier infrared spectroscopy (FT-IR) and Raman spectrometer. Through the DPBF substrate oxidation experiment and the antibacterial ability against S. aureus and E. coli, the photodynamic performance of the material was explored, and the gradient experiment of inorganic salt solution (KI, NaNO2, MgCl2) was designed to explore the effect of its interaction with 1O2 on the antibacterial performance. The results show that the loading of PpIX does not change the basic morphology of the fibrous membrane, but it gives the membrane good substrate oxidation and antibacterial properties (illumination 30 min, the antibacterial efficiency against S. aureus achieves 99.537%). KI, MgCl2and NaNO2solutions all have certain enhancement effects on photodynamic antibacterial mediated by CA/PpIX, among which KI solution has the most obvious enhancement effect. The interaction between the inorganic salt KI and 1O2 produces more free radicals, which can significantly potentiate the photodynamic antibacterial effect. The 100 mmol/L KI solution can potentiate the antibacterial efficiency of the composite fibrous membrane against S. aureus and E. coli to 99.9999%.

Keywords

• protoporphyrin
• electrospinning
• cellulose acetate
• singlet oxygen
• photodynamic antibacterial
• inorganic salt