E3S Web of Conferences (Jan 2020)
The paradigm of induced chemical mutagenesis of Allium sativum L.
Abstract
The scheme of induced chemical mutagenesis of treatment of Allium sativum L. air bulbs of winter garlic bred by FSBSI “Research Institute of Agriculture of Crimea”, grown in a breeding nursery, has been developed. Two chemical mutagens of the first group, capable of transferring alkyd compounds to other plant cells were used in the study. Different concentrations of treatments and monitoring of mutagenesis made it possible to determine the behavior of Allium sativum air bulbs in dynamics: one cloves – M0 mutants (large, medium and small fractions); bulbs – cloves (M1 mutants of the 1st generation); bulbs – cloves (M2 2nd generation mutants). The aim of this work is to assess the morphological changes of Allium sativum L., the effect of chemical mutagens, and the selection of visible mutation-altered forms with valuable economic traits for use in breeding, creating new varieties adapted in Crimea. Monitoring was carried out, the reaction of garlic treated with two chemical mutagens was assessed: DEP (diethyl phosphate) − 0.025; 0.05 and 0.1%, as well as DMS (dimethyl sulfate) 0.02; 0.04 and 0.08% solutions. For control, a sample of Allium sativum L. air bulbs soaked in water was taken. For 2017–2020, different concentrations of mutagens acted selectively on morphometric and economic characteristics, as well as the chemical composition of winter garlic.