Whole planarian chromosome squash

Paul G. Barghouth; Néstor J. Oviedo

STAR Protocols (Mar 2021)

Whole planarian chromosome squash

Paul G. Barghouth,
Néstor J. Oviedo

Affiliations

Paul G. Barghouth: Department of Molecular & Cell Biology, University of California, Merced, Merced, CA, USA; Quantitative and Systems Biology Graduate Program, University of California, Merced, Merced, CA, USA; Corresponding author
Néstor J. Oviedo: Department of Molecular & Cell Biology, University of California, Merced, Merced, CA, USA; Quantitative and Systems Biology Graduate Program, University of California, Merced, Merced, CA, USA; Health Sciences Research Institute, University of California, Merced, Merced, CA, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100257

Abstract

Read online

Summary: Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combination with acetic-orcein, a stain-fixative for chromosomes, this strategy is suitable for animals with friable tissues caused by drug treatment, radiation, and RNA interference phenotypes. The whole planarian squash method presented here is a minimally invasive procedure that facilitates simultaneous analysis of chromosomal integrity in control and experimental animals.For complete details on the use and execution of this protocol, please refer to Peiris et al. (2016).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords