Refashioning benzothiadiazole dye as an activatable nanoprobe for biomarker detection with NIR-II fluorescence/optoacoustic imaging

Junjie Chen; Longqi Chen; Yichang Fang; Fang Zeng; Shuizhu Wu

Cell Reports Physical Science (Feb 2022)

Refashioning benzothiadiazole dye as an activatable nanoprobe for biomarker detection with NIR-II fluorescence/optoacoustic imaging

Junjie Chen,
Longqi Chen,
Yichang Fang,
Fang Zeng,
Shuizhu Wu

Affiliations

Junjie Chen: Biomedical Division, State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, College of Materials Science and Engineering, South China University of Technology, Wushan Road 381, Guangzhou 510640, China
Longqi Chen: Biomedical Division, State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, College of Materials Science and Engineering, South China University of Technology, Wushan Road 381, Guangzhou 510640, China
Yichang Fang: Biomedical Division, State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, College of Materials Science and Engineering, South China University of Technology, Wushan Road 381, Guangzhou 510640, China
Fang Zeng: Biomedical Division, State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, College of Materials Science and Engineering, South China University of Technology, Wushan Road 381, Guangzhou 510640, China; Corresponding author
Shuizhu Wu: Biomedical Division, State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, College of Materials Science and Engineering, South China University of Technology, Wushan Road 381, Guangzhou 510640, China; Corresponding author

Journal volume & issue: Vol. 3, no. 2
p. 100570

Abstract

Read online

Summary: Endogenous nitric oxide is important for living beings; hence, accurate and non-invasive detection of nitric oxide (NO) in situ is of significance. Benzothiadiazole-core chromophores are important near-infrared II (NIR-II) dyes, but such limitations as fluorescence quenching in aqueous media and non-activatable response constitute major barriers for biological applications. To address these issues, here we report an activatable nanoprobe, BNDA@HβCD, with aggregation-induced emission for detecting the biomarker NO. The molecular probe BNDA and 2-hydroxypropyl-β-cyclodextrin (HβCD) form the amphiphilic host-guest complex BNDA-HβCD, which readily forms nanoaggregates in aqueous media and thereby affords the nanoprobe BNDA@HβCD. NO reacts with the probe and generates the activated probe, which displays strong NIR-II fluorescence emission and optoacoustic signals. BNDA@HβCD can detect liver injury and monitor therapeutic outcome in a mouse model by detecting hepatic NO; it can also image NO in soybean sprouts. The results indicate that BNDA@HβCD is a robust tool for detecting NO in both animals and plants.

Published in Cell Reports Physical Science

ISSN: 2666-3864 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Physics
Website: https://www.cell.com/cell-reports-physical-science

About the journal

Abstract

Keywords