Study of the Effect of Carob (Ceratonia siliqua L.) Extract Activity as Antibiotic from UTI

Abstract

Escherichia coli bacterial cells have been collected and selected from(30) patients (most found strain) in urine samples 25 (83.3 %) suffering from infection of urinary tract laid down in Hashimiyah teaching hospital, Babylon during a period from November 2016 to February 2017. The isolated strain diagnosis is confirmed with Vitek 2 system apparatus which perform to identify species level of Escherichia coli isolates .To evaluate the antimicrobial action of the ethanol extract of Carob (Ceratoniasiliqua L.) pods only as well as in mixture with certain drugs (64 µg /ml ampicillin, 32 µg /ml gentamicin, 128 µg /ml amikacin, 8 µg /ml clindamycin.) as the wide usage antibiotics in the treatment of UTI bacterial infections which has led to the emergence and spread of resistant strains. Many studies show that the efficacy of antimicrobials can be improved by combining them with crude plant extracts. The antimicrobial activity of the ethanol extract of pods of Carob (Ceratonia siliqua L.) alone as well as in mixture with some standard antimicrobials has been evaluated using well diffusion method which demonstrates an in-vitro antibacterial activity of the tested extracts against E. Coli bacteria. A combination of the tested extracts( concentration 100%,50%) with antibacterial has increased the activity of the tested antimicrobials. The results reveal the importance of Carob plant extracts when associated with antibiotics to regulator resistance E. Coli bacteria developed as a danger to human health. .

Keywords

• ceratonia siliqua l
• coli
• antibacterial activity
• ampicillin
• gentamicin
• amikacin
• clindamycin drugs
• agar diffusion method