Dynamic analysis of biostimulation of soil polluted by 75000 ppm of waste motor oil

Abstract

Soil polluting by 75000 ppm of waste motor oil (WMO) is a mixture of hydrocarbons (HICO) aliphatic and aromatics, is a concentration which according to NOM-138-SEMARNAT/SSA1-2012 (NOM-138), exceeds the máximum valué permissible of 4400 ppm, this high WMO concentration is inhibin organic matter mineralization and causing soil's fertility. An alternative solution is biostimulation (BIS) of soil polluted by 75000 ppm of WMO, with a detergent (DEGE) that emulsified the WMO, then a mineral solution (MISO), following Phaseolus vulgaris and Vicia sativa powered by Micromonospora echinospora and Penicillium chrysogenum, after 25 days of growing both plants were incorporated and degraded as green manure (GEMA) and vermicompost (VERMI) for an effective mineralization of WMO. The objective of this research was to analyze the dynamics of the BIS of soil polluted by 75000 ppm of WMO to lower valué than the máximum allowed by the NOM-138. In that sense during the BIS, microbial population involved in WMO'oxidation, was counted and by gas chromatography WMO's concentration atthe beginning and end of the assay was determined. Experimental data were analyzed by ANOVA/Tukey. The results indicated that the BIS of the soil impacted by 75000 ppm of WMO decreased it to 1532 ppm, which supports that the biostimulation of the soil with a DEGE, the MISO, GEMA and VERMI enriched it with NH4+, NO3-, PO4-3 and K2O, to induce aerobic heterotrophic microbiota to oxidize the WMO, which was demonstrated by increasing soil's microbial population, pH variation and gas chromatography by disappearance of some aliphatic and aromatics compound of WMO. It is concluded that BIS was worth processing to recover soil impacted by a relatively high concentration of WMO.

Keywords

• soil
• wmo
• microorganisms
• biostimulation
• mineral solution
• p. vulgaris
• v. sativa
• vermicompost
• nom-138
• gas chromatography.