Nanophotonics (Feb 2024)

Highly efficient upconversion photodynamic performance of rare-earth-coupled dual-photosensitizers: ultrafast experiments and excited-state calculations

  • Yang Yubiao,
  • Zhang Lei,
  • Xiao Chao,
  • Huang Zhencheng,
  • Zhao Fuli,
  • Yin Jinchang

DOI
https://doi.org/10.1515/nanoph-2023-0772
Journal volume & issue
Vol. 13, no. 4
pp. 443 – 455

Abstract

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Upconversion photodynamic therapy (UC-PDT), which integrates upconversion nanoparticles (UCNPs) with photosensitizers (PSs), presents a promising advancement in the field of phototherapy. However, despite the extensive studies focused on the design and synthesis of UCNPs, there is a paucity of systematic research on the mechanisms underlying the synergistic upconversion photodynamic effects. Here we have synthesized upconversion core@dotted-shell nanoparticles (CDSNPs) and covalently tethered them with two distinct PSs, thereby constructing a dual-PS UC-PDT system with high synergistic photodynamic performance. To unravel the mechanism underlying the synergism, we employed a combination of quantum mechanical calculations and ultrafast time-resolved spectroscopy techniques. The results indicate that rare earth oxides play a pivotal role in enhancing the intersystem crossing processes of PSs through modulating their excited electronic states. Additionally, Förster resonance energy transfer between two distinct PSs contributes to the amplification of triplet state populations, thus further enhancing the photodynamic effect. In vitro experiments demonstrate that the prepared CDSNPs based dual-PS system exhibits excellent biocompatibility with normal cells and exceptional synergistic photodynamic efficacy against tumor cells upon near-infrared excitation. This research contributes theoretical insights into the design and application of multi-photosensitizer UC-PDT systems, laying the groundwork for more efficient preclinical implementations in the future.

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