Frontiers in Cellular and Infection Microbiology (May 2020)
Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivo
- Jun Tang,
- Jun Tang,
- Jun Tang,
- Jun Tang,
- Jun Tang,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Ya'nan Shi,
- Ya'nan Shi,
- Ya'nan Shi,
- Ya'nan Shi,
- Ya'nan Shi,
- Lingjun Zhan,
- Lingjun Zhan,
- Lingjun Zhan,
- Lingjun Zhan,
- Lingjun Zhan,
- Chuan Qin,
- Chuan Qin,
- Chuan Qin,
- Chuan Qin,
- Chuan Qin
Affiliations
- Jun Tang
- Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, China
- Jun Tang
- NHC Key Laboratory of Human Disease Comparative Medicine, Beijing, China
- Jun Tang
- Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, China
- Jun Tang
- Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, China
- Jun Tang
- Tuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, China
- Zhihao Liu
- Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, China
- Zhihao Liu
- NHC Key Laboratory of Human Disease Comparative Medicine, Beijing, China
- Zhihao Liu
- Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, China
- Zhihao Liu
- Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, China
- Zhihao Liu
- Tuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, China
- Ya'nan Shi
- Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, China
- Ya'nan Shi
- NHC Key Laboratory of Human Disease Comparative Medicine, Beijing, China
- Ya'nan Shi
- Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, China
- Ya'nan Shi
- Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, China
- Ya'nan Shi
- Tuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, China
- Lingjun Zhan
- Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, China
- Lingjun Zhan
- NHC Key Laboratory of Human Disease Comparative Medicine, Beijing, China
- Lingjun Zhan
- Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, China
- Lingjun Zhan
- Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, China
- Lingjun Zhan
- Tuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, China
- Chuan Qin
- Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, China
- Chuan Qin
- NHC Key Laboratory of Human Disease Comparative Medicine, Beijing, China
- Chuan Qin
- Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, China
- Chuan Qin
- Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, China
- Chuan Qin
- Tuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, China
- DOI
- https://doi.org/10.3389/fcimb.2020.00219
- Journal volume & issue
-
Vol. 10
Abstract
Mycobacterium tuberculosis clinical strains usually possess traits different from the laboratory strains like H37Rv, especially those clinical drug resistant strains. With whole genome and transcriptome sequencing, we depicted the feature of two multi-drug resistant Mtb strains in resistance and virulence. Compared with H37Rv, the differential expressed genes (DEGs) of the MDR strains showed featured enrichment in arginine biosynthesis, fatty acid biosynthesis, and metabolism pathway. In the subset of virulence genes, the overlapping DEGs of the MDR strains exhibited downregulation of the cluster in type VII secretion system. In the mice experiment, the MDR strains showed attenuated but distinct virulence, both in survival rate and pathology. Taken together, the whole genome and transcriptome analysis could help understand the unique feature of the MDR strains both in resistance and virulence.
Keywords
- whole genome re-sequencing
- RNA-seq
- transcriptome sequencing
- Mycobacterium tuberculosis
- multi-drug resistant
- virulence
