Circulating Levels of Soluble Receptor Activator of NF-κB Ligand and Matrix Metalloproteinase 3 (and Their Antagonists) in Asian Indian Patients with Ankylosing Spondylitis

Abstract

Background. Bone loss in ankylosing spondylitis may be related to inflammation. Data from previous studies on circulating levels of sRANKL, OPG, MMP3, and TIMP is inconsistent; thus this study is planned to look at this aspect in Asian Indian patients. Methods. Cross-sectional study included patients with ankylosing spondylitis and age- and gender-similar controls. Serum levels of sRANKL, OPG, MMP-3, and TIMP-1 were measured by ELISA. Results. Included 85 patients (M : F = 82 : 3) having mean age (±SD) 33.0±10.0 years and disease duration 11.3±7.3 years. BASDAI, BASFI, BASMI, and ESR were 4.0±2.2, 3.9±2.8, 3.0±2.8, and 59.2±31.2, respectively. Patients had higher mean (±SD) OPG level (649.7±286.8, 389.3±244.8 pg/mL, P<0.001). However, there was no difference in sRANKL (349.2±872.0, 554.7±1850.1, P=ns). Serum MMP-3 (91.4±84.7, 55.9±37.1 ng/mL, P<0.01) and TIMP-1 (520.6±450.7, 296.5±114.2 ng/mL, P<0.001) levels were higher in patients; however, there was no difference in MMP-3/TIMP-1 ratio. Conclusion. Circulating levels of OPG were higher; however, there was no difference in sRANKL in Asian Indian ankylosing spondylitis patients. Although both MMP-3 and TIMP-1 were raised, their ratio was not different from that of controls.