Bacillus subtilis-Fermented Products Ameliorate the Growth Performance, Alleviate Intestinal Inflammatory Gene Expression, and Modulate Cecal Microbiota Community in Broilers during the Starter Phase under Dextran Sulfate Sodium Challenge

Abstract

The aim of this study was to evaluate the effects of B. subtilis-fermented products (BSFP) on growth performance, intestinal inflammatory gene expression, and cecal microbiota community in broilers challenged with dextran sulfate sodium (DSS) in a 14-day experiment. A total of 32, 1-day-old male broiler chickens (Ross 308), were randomly divided into four groups of eight birds per group and reared individually (n＝8). The treatments consisted of a control diet without supplementation and DSS challenge, control diet plus 1.5% DSS, control diet plus 1 g/kg BSFP in combination with 1.5% DSS, and control diet plus 3 g/kg of BSFP in combination with 1.5% DSS. The results showed that BSFP supplementation (1 and 3 g/kg) partially improved body weight and average daily gain in broilers under DSS challenge. Relative to DSS treatment alone, BSFP supplementation dose-dependently increased the body weight of broilers at 7 days of age, with the average daily gain being at 1 to 7 days of age. BSFP supplementation (1 and 3 g/kg) alleviated intestinal inflammatory gene expression in broilers under DSS challenge. The richness and evenness of bacterial species in cecal digesta increased in a dose-dependent manner in the groups treated with BSFP (1 and 3 g/kg) in combination with DSS challenge, compared with the control group. Unweighted principal coordinate analysis indicated distinct clusters separating the group treated with 3 g/kg of BSFP in combination with DSS challenge from the other three groups. The abundance of short-chain fatty acid-producing bacteria (genus Ruminococcaceae_unclassified) increased and that of mucin-degrading bacteria (genus Ruminococcus torques group) decreased in the cecal digesta of broilers fed 3 g/kg of BSFP, compared with the control group. In conclusion, BSFP supplementation dose-dependently improved growth performance, reduced gut inflammation, and regulated the cecal microbiota of broilers exposed to DSS challenge during the starter phase.

Keywords

• bacillus subtilis
• broiler
• fermented product
• dextran sulfate sodium
• microbiota