METHOD OF ESTIMATION OF THE INFLUENCE OF CHEMICAL AND PHYSICAL FACTORS ON BIOPOLYMERS BY THE TEXTURES OF THEIR FILMS

Abstract

Subject and purpose. In this paper, we studied the relationship of the structural and aggregation state of biopolymers with the quantitative characteristics of the textures of the films formed during the drying of biopolymer solutions. Methods and methodology. The structural changes of the biopolymer were determined by IR, UV and fluorescence spectroscopy, the size and surface potential of biopolymer particles were determined by dynamic light scattering, the state of the water environment was determined by microwave dielectrometry. To obtain films, saline solutions of calf thymus Na-DNA, bovine serum albumin (BSA) or human serum albumin (HSA) were dried in a glass cuvette under thermostatically controlled conditions. For the numerical characterization of textures, the relative area, fractal dimension and characteristics of zigzag patterns were used. Results. The paper presents a mini-review of the results during the development of a method for assessing the influence of biologically active substances (inorganic and organic) and physical factors (temperature and gamma irradiation) on biopolymers by the changes in texture parameters. It is shown that the formation of zigzag structures is sensitive to the structural and aggregation effects of biologically active substances and physical factors, and requires the presence of chloride ions. Conclusion. A method for assessing the influence of the corresponding factors by the changes in texture parameters is proposed. The presented results clarify the role of biopolymer, as well as salt cations and anions, in the formation of textures (in particular, zigzag structures) on the surface of films, and show the relationship of structural changes and aggregation of biopolymer with quantitative characteristics of zigzag patterns.

Keywords

• biopolymer
• bovine serum albumin
• dna
• texture
• water-salt solution
• zigzag pattern