PLoS ONE (Mar 2011)

Functional expression of the extracellular calcium sensing receptor (CaSR) in equine umbilical cord matrix size-sieved stem cells.

  • Nicola Antonio Martino,
  • Anna Lange-Consiglio,
  • Fausto Cremonesi,
  • Luisa Valentini,
  • Michele Caira,
  • Antonio Ciro Guaricci,
  • Barbara Ambruosi,
  • Raffaele Luigi Sciorsci,
  • Giovanni Michele Lacalandra,
  • Stephan Joel Reshkin,
  • Maria Elena Dell'Aquila

DOI
https://doi.org/10.1371/journal.pone.0017714
Journal volume & issue
Vol. 6, no. 3
p. e17714

Abstract

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BackgroundThe present study investigates the effects of high external calcium concentration ([Ca(2+)](o)) and the calcimimetic NPS R-467, a known calcium-sensing receptor (CaSR) agonist, on growth/proliferation of two equine size-sieved umbilical cord matrix mesenchymal stem cell (eUCM-MSC) lines. The involvement of CaSR on observed cell response was analyzed at both the mRNA and protein level.Methodology/principal findingsA large (>8 µm in diameter) and a small (Conclusions/significanceIn conclusion, calcium and the calcimimetic NPS R-467 reduce CaSR mRNA expression and stimulate cell growth/proliferation in eUCM-MSC. Their use as components of media for eUCM-MSC culture could be beneficial to obtain enough cells for down-stream purposes.