Reavaliação da suscetibilidade de Candida à anfotericina B: estudo comparativo com isolados de três hospitais do estado do Rio Grande do Sul Reevaluation of Candida susceptibility to amphotericin B: comparative study using isolates from three hospitals in the state of Rio Grande do Sul

Abstract

Comparou-se a suscetibilidade à anfotericina B de Candida spp isoladas de candidemias, sendo: 41 do Hospital Universitário de Santa Maria, 56 do Hospital de Clínicas de Porto Alegre e 47 da Santa Casa, Complexo Hospitalar de Porto Alegre. Os testes foram baseados no documento M27-A2 do Clinical Laboratory Standards Institute. Todavia, foram empregadas 20 concentrações de anfotericina B, variáveis entre 0,1 e 2µg/ml. Os testes foram realizados nos meios RPMI 1640 com glicose, antibiotic medium 3 e yeast nitrogen base dextrosado. O caldo antibiotic medium 3 gerou amplas faixas de concentrações inibitórias mínimas e concentrações fungicidas mínimas quando comparado aos demais. As variações de suscetibilidade entre os hospitais foram melhor detectadas no antibiotic médium 3; os isolados do Hospital Universitário de Santa Maria evidenciaram menor sensibilidade do que os da Santa Casa, Complexo Hospitalar de Porto Alegre (p Susceptibility to amphotericin B was compared between isolates of Candida spp that were obtained from candidemia cases as follows: 41 from Hospital Universitário de Santa Maria, 56 from Hospital de Clínicas, Porto Alegre, and 47 from the Santa Casa hospital complex, Porto Alegre. The tests were based on the document M27-A2 from the Clinical Laboratory Standards Institute, but with 20 concentrations of amphotericin B ranging from 0.1 to 2mg/ml. The tests were carried out using RPMI 1640 medium with glucose, antibiotic medium 3 and yeast nitrogen base-dextrose. The antibiotic medium 3 broth generated wide ranges of minimum inhibitory concentrations and minimum fungicidal concentrations in relation to the other agents. The variations in susceptibility between the hospitals were best detected in antibiotic medium 3. The isolates from Hospital Universitário de Santa Maria showed lower susceptibility than did those from the Santa Casa hospital complex, Porto Alegre (p < 0.05). The causes of the susceptibility variations were not assessed but they indicate the need for surveillance regarding the susceptibility to amphotericin B.

Keywords

• Suscetibilidade
• Anfotericina B
• Candida
• Candidemia
• Susceptibility
• Amphotericin B
• Candida
• Candidemia