Морфологія (Jan 2010)
Influense of the effect of experimental diabetes mellitus on the protein expression of apoptotic regulators Bcl-2 and p53 in the lymphoid follicles of the spleen
Abstract
The problem of investigation of molecular mechanisms of apoptosis and its role in autoimmune disease, includingdiabetes mellitus, became one of the most difficult and actual among the medical sciences last years. Under the experimentthe effect of experimental diabetes mellitus on the intensity of protein expression of apoptotic regulators Bcl-2 and p53 in thespleen of rats was investigated. To detect Bcl-2 and p53 positive-cells we used immunohistochemical technique of indirectimmunofluorescence with monoclonal antibodies to Bcl-2 and p53. It was found that the development of experimentaldiabetes mellitus is accompanied by unidirectional tendency to increase the number of p53- and Bcl-2-positive cells inlymphoid follicles of the spleen with a preferential decrease in the concentration of these protein in immune cells.
