Chaperone‐mediated autophagy affects tumor cell proliferation and cisplatin resistance in esophageal squamous cell carcinoma

Dujuan Cao; Danyang Shan; Wenli Yan; Zifang Zhang; Qianqian Song; Yuanyuan Jiang; Xinmiao Zhang; Zhongxian Zhang; Zhimin Wang; Yaohe Wang; Shuangshuang Lu

doi:10.1111/1759-7714.13849

Thoracic Cancer (Apr 2021)

Chaperone‐mediated autophagy affects tumor cell proliferation and cisplatin resistance in esophageal squamous cell carcinoma

Dujuan Cao,
Danyang Shan,
Wenli Yan,
Zifang Zhang,
Qianqian Song,
Yuanyuan Jiang,
Xinmiao Zhang,
Zhongxian Zhang,
Zhimin Wang,
Yaohe Wang,
Shuangshuang Lu

Affiliations

Dujuan Cao: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Danyang Shan: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Wenli Yan: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Zifang Zhang: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Qianqian Song: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Yuanyuan Jiang: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Xinmiao Zhang: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Zhongxian Zhang: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Zhimin Wang: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Yaohe Wang: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China
Shuangshuang Lu: National Center for International Research in Cell and Gene Therapy, Sino‐British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Academy of Medical Sciences Zhengzhou University Zhengzhou China

DOI: https://doi.org/10.1111/1759-7714.13849
Journal volume & issue: Vol. 12, no. 7
pp. 1048 – 1057

Abstract

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Abstract Background Chaperone‐mediated autophagy (CMA) is a lysosomal degradation pathway of selective soluble proteins. Lysosomal membrane associated protein 2a (LAMP2a) is the lysosomal membrane receptor of CMA and influences CMA activity. Although it has been suggested that higher expression of LAMP2a is associated with more advanced tumor node metastasis (TNM) stages and shorter survival time in patients with esophageal squamous cell carcinoma (ESCC), the underlying mechanism has not been known yet. Methods In this study, we modulated the activity of CMA through LAMP2a or small molecular compounds in human ESCC cells to investigate its role in ESCC. Results We found that down‐regulating the activity of CMA could inhibit the proliferation and colony formation of ESCC cells as well as increase their sensitivity to cisplatin. Conclusions Our results promote better understanding of how CMA affects human ESCC and provide a new therapeutic target against ESCC through down‐regulating LAMP2a.

Published in Thoracic Cancer

ISSN: 1759-7706 (Print); 1759-7714 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://onlinelibrary.wiley.com/journal/17597714

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