Development of a solid-phase microextraction method for determination of hexanal in mayonnaise-type soy dressing

Abstract

The aim of this study was to develop a headspace-solid phase microextraction (HS-SPME) method combined with gas chromatography for the determination of hexanal as an indicator of the oxidative changes in mayonnaise-type soy dressing. The identification and quantification of hexanal was done by gas chromatography with a mass selective detector (GC-MS). The method was optimized in terms of the type of fiber, temperature and extraction time, using a response surface design, and the hexanal content as response variable. The method was established with the polydimethylsiloxane-divinylbenzene fiber (PDMS/DVB), 0.3 g of sample, 10 min of pre-extraction, 35 min of extraction at 40 oC and 2 min of desorption at 250 oC. The validation of the developed method was carried out considering linearity, detection and quantification limits, and precision. Validation for the hexanal content technique showed that the method has a linear response in the range of 1 to 3000 ng/g of hexanal, a limit of detection of 1.06 ng/g, a limit of quantification of 3.53 ng/g, 3.3% average repeatability and 97.7% recovery