Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy

Jie Li; Pengjun Wang; Lu-Yang Wang; Yaqin Wu; Jiping Wang; Dongzhen Yu; Zhengnong Chen; Haibo Shi; Haibo Shi; Shankai Yin; Shankai Yin

doi:10.3389/fnins.2023.1146147

Frontiers in Neuroscience (Jun 2023)

Redistribution of the astrocyte phenotypes in the medial vestibular nuclei after unilateral labyrinthectomy

Jie Li,
Pengjun Wang,
Lu-Yang Wang,
Yaqin Wu,
Jiping Wang,
Dongzhen Yu,
Zhengnong Chen,
Haibo Shi,
Haibo Shi,
Shankai Yin,
Shankai Yin

Affiliations

Jie Li: Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
Pengjun Wang: Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
Lu-Yang Wang: Programs in Neurosciences & Mental Health, SickKids Research Institute and Department of Physiology, University of Toronto, Toronto, ON, Canada
Yaqin Wu: Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
Jiping Wang: Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
Dongzhen Yu: Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
Zhengnong Chen: Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
Haibo Shi: Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
Haibo Shi: Shanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, China
Shankai Yin: Department of Otorhinolaryngology—Head and Neck Surgery, Shanghai Sixth People’s Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China
Shankai Yin: Shanghai Key Laboratory of Sleep Disordered Breathing, Shanghai, China

DOI: https://doi.org/10.3389/fnins.2023.1146147
Journal volume & issue: Vol. 17

Abstract

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Astrocytes are highly heterogeneous and involved in different aspects of fundamental functions in the central nervous system (CNS). However, whether and how this heterogeneous population of cells reacts to the pathophysiological challenge is not well understood. To investigate the response status of astrocytes in the medial vestibular nucleus (MVN) after vestibular loss, we examined the subtypes of astrocytes in MVN using single-cell sequencing technology in a unilateral labyrinthectomy mouse model. We discovered four subtypes of astrocytes in the MVN with each displaying unique gene expression profiles. After unilateral labyrinthectomy, the proportion of the astrocytic subtypes and their transcriptional features on the ipsilateral side of the MVN differ significantly from those on the contralateral side. With new markers to detect and classify the subtypes of astrocytes in the MVN, our findings implicate potential roles of the adaptive changes of astrocyte subtypes in the early vestibular compensation following peripheral vestibular damage to reverse behavioral deficits.

Published in Frontiers in Neuroscience

ISSN: 1662-4548 (Print); 1662-453X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: http://www.frontiersin.org/neuroscience

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