Spatiotemporal Dynamics of Bacterial Community Assembly and Co-Occurrence Patterns in Biological Soil Crusts of Desert Ecosystems

Abstract

Biological soil crusts (BSCs) play a fundamental role in desert ecosystems by stabilizing soil, cycling nutrients, and retaining moisture. However, the assembly processes governing bacterial communities within BSCs remain largely unknown. This study aimed to reveal the spatiotemporal variations in the bacterial community diversity, co-occurrence patterns, and ecological assembly processes of BSCs and their underlying soils across different desert and seasonal conditions. We systematically analyzed the spatial differences in the bacterial diversity, co-occurrence networks, and community assembly processes of BSCs and their underlying soils using samples collected at various soil depths from different BSC types in different deserts. We discovered that BSC type and soil depth were the primary factors driving bacterial community assembly, while seasonal effects were weaker and more indirect, and mainly regulated community dynamics through changes in resource availability and environmental conditions. The underlying soils of moss- and lichen-BSCs exhibited higher bacterial diversity and richness than those of algae BSCs. In contrast, cyano-BSCs exhibited a lower diversity, but Cyanobacteria demonstrated the highest photosynthetic function. Among the different deserts, the community assembly of samples from the eastern Inner Mongolia deserts was largely influenced by environmental selection, whereas stochastic processes were more prominent in the central and western desert regions. A β-nearest taxon index (βNTI) analysis indicated that stochastic processes were dominant in surface BSC samples, while environmental selection played a stronger role in deeper layers. A co-occurrence network analysis revealed that surface BSC samples had a high degree of network connectivity, with those from moss- and lichen-BSCs being particularly high, and they also exhibited high modularity and local clustering that promoted the functional stability of the microbial communities. This study revealed the integrated effects of soil depth, BSC type, desert type, and resource availability on microbial community assembly in desert ecosystems. These findings provide a theoretical basis for the microbial management of BSCs and scientific insights to support restoration strategies in desert ecosystems.

Keywords

• biological soil crusts
• bacterial communities
• β-NTI
• community assembly
• environmental filtering
• network analysis