Anti-Listerial Activity of Bacteriocin-like Inhibitory Substance Produced by <i>Enterococcus lactis</i> LBM BT2 Using Alternative Medium with Sugarcane Molasses
Taciana Freire de Oliveira,
Taís Mayumi Kuniyoshi,
Elionio Galvão Frota,
Sebastián Bermúdez-Puga,
Letícia Naomy Sakaue,
Luara Lucena Cassiano,
Leonardo Tachibana,
Rosane Aparecida Moniz Piccoli,
Attilio Converti,
Ricardo Pinheiro de Souza Oliveira
Affiliations
Taciana Freire de Oliveira
Laboratory of Microbial Biomolecules, School of Pharmaceutical Sciences, University of São Paulo, Rua do Lago, 250, São Paulo 05508-000, Brazil
Taís Mayumi Kuniyoshi
Laboratory of Microbial Biomolecules, School of Pharmaceutical Sciences, University of São Paulo, Rua do Lago, 250, São Paulo 05508-000, Brazil
Elionio Galvão Frota
Laboratory of Microbial Biomolecules, School of Pharmaceutical Sciences, University of São Paulo, Rua do Lago, 250, São Paulo 05508-000, Brazil
Sebastián Bermúdez-Puga
Laboratory of Microbial Biomolecules, School of Pharmaceutical Sciences, University of São Paulo, Rua do Lago, 250, São Paulo 05508-000, Brazil
Letícia Naomy Sakaue
Laboratory of Microbial Biomolecules, School of Pharmaceutical Sciences, University of São Paulo, Rua do Lago, 250, São Paulo 05508-000, Brazil
Luara Lucena Cassiano
Aquaculture Research Center, Scientific Research of Fisheries Institute, APTA, SAA, Av. Conselheiro Rodrigues Alves, 1252, São Paulo 04014-002, Brazil
Leonardo Tachibana
Aquaculture Research Center, Scientific Research of Fisheries Institute, APTA, SAA, Av. Conselheiro Rodrigues Alves, 1252, São Paulo 04014-002, Brazil
Rosane Aparecida Moniz Piccoli
Bionanomanufacturing Nucleus, Institute for Technological Research (IPT), Av. Prof. Almeida Prado, 532, São Paulo 05508-901, Brazil
Attilio Converti
Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy
Ricardo Pinheiro de Souza Oliveira
Laboratory of Microbial Biomolecules, School of Pharmaceutical Sciences, University of São Paulo, Rua do Lago, 250, São Paulo 05508-000, Brazil
Listeria monocytogenes is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, thus reaching final products by cross-contamination. With the growing demand for clean-label products, the search for natural antimicrobials as biopreservants, such as bacteriocins, has shown promising potential. In this context, this study aimed to evaluate the anti-listerial action of bacteriocins produced by Enterococcus lactis LBM BT2 in an alternative medium containing sugarcane molasses (SCM). Molecular analyses were carried out to characterize the strain, including the presence of bacteriocin-related genes. In the kinetic study on SCM medium E. lactis, LBM BT2 showed biomass and bacteriocin productions similar to those observed on a sucrose-based medium (control), highlighting the potential of the sugarcane molasses as a low-cost substrate. Stability tests revealed that the molecule remained active in wide ranges of pH (4–10) and temperature (60–100 °C). Furthermore, the proteolytic treatment reduced the biomolecule’s antimicrobial activity, highlighting its proteinaceous nature. After primary purification by salting out and tangential flow filtration, the bacteriocin-like inhibitory substance (BLIS) showed bacteriostatic activity on suspended L. monocytogenes cells and against biofilm formation at a concentration of 0.625 mg/mL. These results demonstrate the potential of the produced BLIS as a biopreservative in the food industry.
