A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies

Adrian Creanga; Rebecca A. Gillespie; Brian E. Fisher; Sarah F. Andrews; Julia Lederhofer; Christina Yap; Liam Hatch; Tyler Stephens; Yaroslav Tsybovsky; Michelle C. Crank; Julie E. Ledgerwood; Adrian B. McDermott; John R. Mascola; Barney S. Graham; Masaru Kanekiyo

doi:10.1038/s41467-021-21954-2

Nature Communications (Mar 2021)

A comprehensive influenza reporter virus panel for high-throughput deep profiling of neutralizing antibodies

Adrian Creanga,
Rebecca A. Gillespie,
Brian E. Fisher,
Sarah F. Andrews,
Julia Lederhofer,
Christina Yap,
Liam Hatch,
Tyler Stephens,
Yaroslav Tsybovsky,
Michelle C. Crank,
Julie E. Ledgerwood,
Adrian B. McDermott,
John R. Mascola,
Barney S. Graham,
Masaru Kanekiyo

Affiliations

Adrian Creanga: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Rebecca A. Gillespie: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Brian E. Fisher: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Sarah F. Andrews: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Julia Lederhofer: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Christina Yap: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Liam Hatch: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Tyler Stephens: Electron Microscopy Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute
Yaroslav Tsybovsky: Electron Microscopy Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute
Michelle C. Crank: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Julie E. Ledgerwood: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Adrian B. McDermott: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
John R. Mascola: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Barney S. Graham: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Masaru Kanekiyo: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health

DOI: https://doi.org/10.1038/s41467-021-21954-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Understanding the human antibody response to influenza A virus strains is important for vaccine development. Here, Creanga et al. generate a panel of 55 replication-deficient reporter viruses representing diversity of human H1N1 and H3N2, and pandemic subtypes and characterize the neutralization profile of 24 antibodies and polyclonal sera.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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