Department of Biology, University of Copenhagen, Copenhagen, DenmarkKey Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China
Xiuli Hao
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China
Freja Lüthje
Department of Biology, University of Copenhagen, Copenhagen, Denmark
Nadezhda German
Department of Pharmaceutical Sciences, Texas Tech University Health Sciences Center, Amarillo, TX, USA
Xuanji Li
Department of Biology, University of Copenhagen, Copenhagen, Denmark
Fuyi Huang
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China
Javan Kisaka
Department of Chemistry, Western Michigan University, Kalamazoo, MI, USA
David Huffman
Department of Chemistry, Western Michigan University, Kalamazoo, MI, USADepartment of Plant and Environmental Sciences, University of Copenhagen, MO, USA
Hend Alwathnani
Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia
Yong-Guan Zhu
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China
Christopher Rensing
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaJ. Craig Venter Institute, La Jolla, CA, USA, Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture & Forestry University, Fuzhou, China
We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested bacteria were killed by gentamicin. Gentamicin is used because it does not penetrate into eukaryotic cells allowing the ingested bacteria to survive the antibiotic treatment, whereas bacteria outside the amoebal cells are killed.
