Injection Parameters Affect Cell Viability and Implant Volumes in Automated Cell Delivery for the Brain

Abstract

The technique of central nervous system cell implantation can affect the outcome of preclinical or clinical studies. Our goal was to evaluate the impact of various injection parameters that may be of consequence during the delivery of solute-suspended cells. These parameters included ( 1 ) the type and concentration of cells used for implantation, ( 2 ) the rate at which cells are injected (flow rate), ( 3 ) the acceleration of the delivery device, ( 4 ) the period of time between cell loading and injection into the CNS (delay), and ( 5 ) the length and gauge of the needle used to deliver the cells. Neural progenitor cells (NPCs) and bone marrow stromal cells (BMSCs) were injected an automated device. These parameters were assessed in relation to their effect on the volume of cells injected and cell viability. Longer and thinner cannulae and higher cell concentrations were detrimental for cell delivery. Devices and techniques that optimize these parameters should be of benefit.