Frontiers in Bioengineering and Biotechnology (Nov 2021)
Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening
- Lei Zeng,
- Lei Zeng,
- Lei Zeng,
- Yanqi Bi,
- Yanqi Bi,
- Yanqi Bi,
- Pengfei Guo,
- Pengfei Guo,
- Pengfei Guo,
- Yali Bi,
- Yali Bi,
- Yali Bi,
- Tiantian Wang,
- Tiantian Wang,
- Tiantian Wang,
- Liang Dong,
- Liang Dong,
- Liang Dong,
- Fangzhong Wang,
- Fangzhong Wang,
- Lei Chen,
- Lei Chen,
- Lei Chen,
- Weiwen Zhang,
- Weiwen Zhang,
- Weiwen Zhang,
- Weiwen Zhang
Affiliations
- Lei Zeng
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Lei Zeng
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
- Lei Zeng
- SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
- Yanqi Bi
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Yanqi Bi
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
- Yanqi Bi
- SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
- Pengfei Guo
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Pengfei Guo
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
- Pengfei Guo
- SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
- Yali Bi
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Yali Bi
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
- Yali Bi
- SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
- Tiantian Wang
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Tiantian Wang
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
- Tiantian Wang
- SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
- Liang Dong
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Liang Dong
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
- Liang Dong
- SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
- Fangzhong Wang
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Fangzhong Wang
- Center for Biosafety Research and Strategy, Tianjin University, Tianjin, China
- Lei Chen
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Lei Chen
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
- Lei Chen
- SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
- Weiwen Zhang
- Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, China
- Weiwen Zhang
- Center for Biosafety Research and Strategy, Tianjin University, Tianjin, China
- Weiwen Zhang
- Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
- Weiwen Zhang
- SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
- DOI
- https://doi.org/10.3389/fbioe.2021.738052
- Journal volume & issue
-
Vol. 9
Abstract
High DHA production cost caused by low DHA titer and productivity of the current Schizochytrium strains is a bottleneck for its application in competition with traditional fish-oil based approach. In this study, atmospheric and room-temperature plasma with iodoacetic acid and dehydroepiandrosterone screening led to three mutants, 6–8, 6–16 and 6–23 all with increased growth and DHA accumulations. A LC/MS metabolomic analysis revealed the increased metabolism in PPP and EMP as well as the decreased TCA cycle might be relevant to the increased growth and DHA biosynthesis in the mutants. Finally, the mutant 6–23, which achieved the highest growth and DHA accumulation among all mutants, was evaluated in a 5 L fermentor. The results showed that the DHA concentration and productivity in mutant 6–23 were 41.4 g/L and 430.7 mg/L/h in fermentation for 96 h, respectively, which is the highest reported so far in literature. The study provides a novel strain improvement strategy for DHA-producing Schizochytrium.
Keywords
