Nature Communications (Jan 2020)

Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform

  • Christian Munck,
  • Ravi U. Sheth,
  • Daniel E. Freedberg,
  • Harris H. Wang

DOI
https://doi.org/10.1038/s41467-019-14012-5
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

Read online

Horizontal gene transfer (HGT) in complex bacterial communities has been mostly studied using metagenomic analyses. Here, the authors develop an E. coli CRISPR-Cas spacer acquisition platform that allows real-time recording of HGT events at nucleotide-resolution, identifying diverse DNA transfer events in human clinical fecal samples.